Remodelling of adult cardiac tissue subjected to physiological and pathological mechanical load in vitro.

ABSTRACT:

Aims

Cardiac remodelling is the process by which the heart adapts to its environment. Mechanical load is a major driver of remodelling. Cardiac tissue culture has been frequently employed for in vitro studies of load-induced remodelling; however, current in vitro protocols (e.g. cyclic stretch, isometric load, and auxotonic load) are oversimplified and do not accurately capture the dynamic sequence of mechanical conformational changes experienced by the heart in vivo. This limits translational scope and relevance of findings.

Methods and results

We developed a novel methodology to study chronic load in vitro. We first developed a bioreactor that can recreate the electromechanical events of in vivo pressure-volume loops as in vitro force-length loops. We then used the bioreac

SUBMITTER: Pitoulis FG 

PROVIDER: S-EPMC8859636 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature