Introducing an Artificial Deazaflavin Cofactor in Escherichia coli and Saccharomyces cerevisiae.

ABSTRACT: Deazaflavin-dependent whole-cell conversions in well-studied and industrially relevant microorganisms such as Escherichia coli and Saccharomyces cerevisiae have high potential for the biocatalytic production of valuable compounds. The artificial deazaflavin FOP (FO-5'-phosphate) can functionally substitute the natural deazaflavin F₄₂₀ and can be synthesized in fewer steps, offering a solution to the limited availability of the latter due to its complex (bio)synthesis. Herein we set out to produce FOP in vivo as a scalable FOP production method and as a means for FOP-mediated whole-cell conversions. Heterologous expression of the riboflavin kinase from Schizosaccharomyces pombe enabled in vivo phosphorylation of FO, which was supplied by either organic synthe

SUBMITTER: Lee M 

PROVIDER: S-EPMC8859854 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature