Single-cell RNA-sequencing analysis of the ciliary epithelium and contiguous tissues in the mouse eye.

ABSTRACT: The ciliary epithelium plays a central role in ocular homeostasis but cells of the pigmented and non-pigmented layers are difficult to isolate physically and study. Here we used single-cell RNA-sequencing (scRNA-seq) to analyze the transcriptional signatures of cells harvested from the ciliary body and contiguous tissues. Microdissected tissue was dissociated by collagenase digestion and the transcriptomes of individual cells were obtained using a droplet-based scRNA-seq approach. In situ hybridization was used to verify the expression patterns of selected differentially-expressed genes. High quality transcriptomes were obtained from 10,024 cells and unsupervised clustering distinguished 22 cell types. Although efforts were made to specifically isolate the ciliary body, approximately half

SUBMITTER: Youkilis JC 

PROVIDER: S-EPMC8860325 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature