Identification, functional characterization, assembly and structure of ToxIN type III toxin-antitoxin complex from E. coli.

ABSTRACT: Toxin-antitoxin (TA) systems are proposed to play crucial roles in bacterial growth under stress conditions such as phage infection. The type III TA systems consist of a protein toxin whose activity is inhibited by a noncoding RNA antitoxin. The toxin is an endoribonuclease, while the antitoxin consists of multiple repeats of RNA. The toxin assembles with the individual antitoxin repeats into a cyclic complex in which the antitoxin forms a pseudoknot structure. While structure and functions of some type III TA systems are characterized, the complex assembly process is not well understood. Using bioinformatics analysis, we have identified type III TA systems belonging to the ToxIN family across different Escherichia coli strains and found them to be clustered into at least five distinct clu

SUBMITTER: Manikandan P 

PROVIDER: S-EPMC8860590 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature