Development of an efficient wastewater testing protocol for high-throughput country-wide SARS-CoV-2 monitoring.

ABSTRACT: Wastewater-based surveillance has been widely used as a non-intrusive tool to monitor population-level transmission of COVID-19. Although various approaches are available to concentrate viruses from wastewater samples, scalable methods remain limited. Here, we sought to identify and evaluate SARS-CoV-2 virus concentration protocols for high-throughput wastewater testing. A total of twelve protocols for polyethylene glycol (PEG) precipitation and four protocols for ultrafiltration-based approaches were evaluated across two phases. The first phase entailed an initial evaluation using a small sample set, while the second phase further evaluated five protocols using wastewater samples of varying SARS-CoV-2 concentrations. Permutations in the pre-concentration, virus concentration and RNA extra

SUBMITTER: Mailepessov D 

PROVIDER: S-EPMC8860745 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature