A Three-Dimensional Mechanical Loading Model of Human Osteocytes in Their Native Matrix.

ABSTRACT: Osteocytes are mechanosensory cells which are embedded in calcified collagenous matrix. The specific native matrix of osteocytes affects their regulatory activity, i.e., transmission of signaling molecules to osteoclasts and/or osteoblasts, in the mechanical adaptation of bone. Unfortunately, no existing in vitro model of cortical bone is currently available to study the mechanosensory function of human osteocytes in their native matrix. Therefore, we aimed to develop an in vitro three-dimensional mechanical loading model of human osteocytes in their native matrix. Human cortical bone explants containing osteocytes in their three-dimensional native matrix were cultured and mechanically loaded by three-point bending using a custom-made loading apparatus generating sinusoidal displacement. O

SUBMITTER: Zhang C 

PROVIDER: S-EPMC8860829 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature