Conformational dynamics promotes disordered regions from function-dispensable to essential in evolved site-specific DNA recombinases.

ABSTRACT: Protein intrinsically disordered regions (IDRs) play pivotal roles in molecular recognition and regulatory processes through structural disorder-to-order transitions. To understand and exploit the distinctive functional implications of IDRs and to unravel the underlying molecular mechanisms, structural disorder-to-function relationships need to be deciphered. The DNA site-specific recombinase system Cre/loxP represents an attractive model to investigate functional molecular mechanisms of IDRs. Cre contains a functionally dispensable disordered N-terminal tail, which becomes indispensable in the evolved Tre/loxLTR recombinase system. The difficulty to experimentally obtain structural information about this tail has so far precluded any mechanistic study on its involvement in DNA recombinati

SUBMITTER: Guillen-Pingarron C 

PROVIDER: S-EPMC8860914 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature