Human Stool Preservation Impacts Taxonomic Profiles in 16S Metagenomics Studies.

ABSTRACT: Microbiotas play critical roles in human health, yet in most cases scientists lack standardized and reproducible methods from collection and preservation of samples, as well as the choice of omic analysis, up to the data processing. To date, stool sample preservation remains a source of technological bias in metagenomic sequencing, despite newly developed storage solutions. Here, we conducted a comparative study of 10 storage methods for human stool over a 14-day period of storage at fluctuating temperatures. We first compared the performance of each stabilizer with observed bacterial composition variation within the same specimen. Then, we identified the nature of the observed variations to determine which bacterial populations were more impacted by the stabilizer. We found that DNA stabi

SUBMITTER: Plauzolles A 

PROVIDER: S-EPMC8860989 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature