Enhanced O-GlcNAc modification induced by the RAS/MAPK/CDK1 pathway is required for SOX2 protein expression and generation of cancer stem cells.

ABSTRACT: Cancer stem cells (CSCs) have tumour initiation, self-renewal, and long-term tumour repopulation properties, and it is postulated that differentiated somatic cells can be reprogrammed to CSCs by oncogenic signals. We previously showed that oncogenic HRAS^V12 conferred tumour initiation capacity in tumour suppressor p53-deficient (p53^-/-) primary mouse embryonic fibroblasts (MEFs) through transcription factor NF-κB-mediated enhancement of glucose uptake; however, the underlying mechanisms of RAS oncogene-induced CSC reprogramming have not been elucidated. Here, we found that the expression of the reprogramming factor SOX2 was induced by HRAS^V12 in p53^-/- MEFs. Moreover, gene knockout studies revealed that SOX2 is an essential factor for the generat

SUBMITTER: Shimizu M 

PROVIDER: S-EPMC8861017 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature