Physiological and transcriptome analysis reveals the differences in nitrate content between lamina and midrib of flue-cured tobacco.

ABSTRACT: Nitrate is an important precursor of tobacco-specific nitrosamines (TSNAs) and a remarkable difference in nitrate accumulation between lamina and midrib of flue-cured tobacco has long been observed. However, the physiological and molecular mechanisms underpinning this difference remain poorly understood. In this study, physiological and genetic factors impacting nitrate accumulation were identified in pot experiments using flue-cured tobacco K326 with contrasting nitrate content between lamina and midrib. The results showed that three times higher NO₃-N content was observed in midrib than that in the lamina, along with lower pigment, NH₄-N content, nitrate reductase activity (NRA), sucrose synthetase activity (SSA), and glutamine synthetase activity (GSA) in midrib. T

SUBMITTER: Feng Y 

PROVIDER: S-EPMC8861034 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature