Multichannel optogenetics combined with laminar recordings for ultra-controlled neuronal interrogation.

ABSTRACT: Simultaneous large-scale recordings and optogenetic interventions may hold the key to deciphering the fast-paced and multifaceted dialogue between neurons that sustains brain function. Here we have taken advantage of thin, cell-sized, optical fibers for minimally invasive optogenetics and flexible implantations. We describe a simple procedure for making those fibers side-emitting with a Lambertian emission distribution. Here we combined those fibers with silicon probes to achieve high-quality recordings and ultrafast multichannel optogenetic inhibition. Furthermore, we developed a multi-channel optical commutator and general-purpose patch-cord for flexible experiments. We demonstrate that our framework allows to conduct simultaneous laminar recordings and multifiber stimulations, 3D optoge

SUBMITTER: Eriksson D 

PROVIDER: S-EPMC8861070 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature