Isolation, molecular identification of lipid-producing Rhodotorula diobovata: optimization of lipid accumulation for biodiesel production.

ABSTRACT:

Background

The increased demand for oil and fats to satisfy the ever-increasing human needs has enhanced the research in this field. Single-cell oils or microbial lipids produced by oleaginous microorganisms are being utilized as an alternative to traditional oil sources. Oleaginous yeasts can accumulate lipids above 20% of their biomass when they are grown under controlled conditions.

Results

In the present study, sixty-five yeasts were isolated from different sources. Using Sudan Black B staining technique, five yeast isolates were selected. Under nitrogen-limited cultivation conditions, the Co1 isolate was the best lipid accumulation potential of 39.79%. Isolate (Co1) was characterized morphologically and identified using the ribosomal DNA internal transcribed spacers reg

SUBMITTER: Osman ME 

PROVIDER: S-EPMC8861238 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature