Evaluation of Laser Confocal Raman Spectroscopy as a Non-Invasive Method for Detecting Sperm DNA Contents.

ABSTRACT:

Research question

Is Raman spectroscopy an efficient and accurate method to detect sperm chromosome balance state by DNA content differences?

Design

Semen samples were provided by diploid healthy men, and the analysis parameters met the current World Health Organization standards. The DNA content was assessed by analysis of the corresponding spectra obtained from a laser confocal Raman spectroscope. The sperm sex chromosome information was obtained by fluorescence in situ hybridization (FISH). Comparative analysis was performed between FISH results and Raman spectral analysis results.

Results

Different parts of the sperm head showed different spectral signal intensities, which indicated that there were different chemical components. Standard principal component

SUBMITTER: Li M 

PROVIDER: S-EPMC8861532 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature