Circuit topology analysis of cellular genome reveals signature motifs, conformational heterogeneity, and scaling.

ABSTRACT: Reciprocal regulation of genome topology and function is a fundamental and enduring puzzle in biology. The wealth of data provided by Hi-C libraries offers the opportunity to unravel this relationship. However, there is a need for a comprehensive theoretical framework in order to extract topological information for genome characterization and comparison. Here, we develop a toolbox for topological analysis based on Circuit Topology, allowing for the quantification of inter- and intracellular genomic heterogeneity, at various levels of fold complexity: pairwise contact arrangement, higher-order contact arrangement, and topological fractal dimension. Single-cell Hi-C data were analyzed and characterized based on topological content, revealing not only a strong multiscale heterogeneity but als

SUBMITTER: Scalvini B 

PROVIDER: S-EPMC8861635 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature