Stimuli Induced Uptake of Protein-Like Peptide Brush Polymers.

ABSTRACT: Recently, we presented a strategy for packaging peptides as side-chains in high-density brush polymers. For this globular protein-like polymer (PLP) formulation, therapeutic peptides were shown to resist proteolytic degradation, enter cells efficiently and maintain biological function. In this paper, we establish the role charge plays in dictating the cellular uptake of these peptide formulations, finding that peptides with a net positive charge will enter cells when polymerized, while those formed from anionic or neutral peptides remain outside of cells. Given these findings, we explored whether cellular uptake could be selectively induced by a stimulus. In our design, a cationic peptide is appended to a sequence of charge-neutralizing anionic amino acids through stimuli-responsive cleava

SUBMITTER: Blum AP 

PROVIDER: S-EPMC8861929 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature