Urinary sediment CCL5 messenger RNA as a potential prognostic biomarker of diabetic nephropathy.

ABSTRACT:

Background

Urinary sediment messenger RNAs (mRNAs) have been shown as novel biomarkers of kidney disease. We aimed to identify targeted urinary mRNAs in diabetic nephropathy (DN) based on bioinformatics analysis and clinical validation.

Methods

Microarray studies of DN were searched in the GEO database and Nephroseq platform. Gene modules negatively correlated with estimated glomerular filtration rate (eGFR) were identified by informatics methods. Hub genes were screened within the selected modules. In validation cohorts, a quantitative polymerase chain reaction assay was used to compare the expression levels of candidate mRNAs. Patients with renal biopsy-confirmed DN were then followed up for a median time of 21 months. End-stage renal disease (ESRD) was defined as the prim

SUBMITTER: Feng ST 

PROVIDER: S-EPMC8862108 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature