Ibuprofen inhibits key genes involved in androgen production in theca-interstitial cells.

ABSTRACT:

Objective

To study the effects of ibuprofen on androgen production, gene expression, and cell viability in rat theca-interstitial cells exposed to the proinflammatory stimuli interleukin-1β (IL-1β) and lipopolysaccharide (LPS).

Design

Animal study.

Setting

University-based research laboratory.

Patients/animals

Theca-interstitial cells were isolated from 30 day old female Sprague Dawley rats.

Interventions

Theca cells were cultured with pro-inflammatory media containing IL-1β and LPS and compared with cells cultured in control media.

Main outcome measures

Androstenedione quantification was performed on conditioned cell culture medium using liquid chromatography-mass spectrometry. Theca cell viability was assessed using PrestoBl

SUBMITTER: Fox CW 

PROVIDER: S-EPMC8862173 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature