Metaproteomic profiling of fungal gut colonization in gnotobiotic mice.

ABSTRACT:

Background

Eukaryotic microbes can modulate mammalian host health and disease states, yet the molecular contribution of gut fungi remains nascent. We previously showed that mice exclusively colonised with fungi displayed increased sensitivity to allergic airway inflammation and had fecal metabolite profiles similar to germ-free mice. This marginal effect on the host metabolome suggested that fungi do not primarily use metabolites to modulate the host immune system.

Methods

To describe functional changes attributed to fungal colonisation, we performed mass spectrometry-based analyses of feces (Label-Free Quantitative; LFQ) and the small intestine (labeling with Tandem Mass Tag; TMT) of gnotobiotic mice colonised with defined consortia of twelve bacterial species, five fungal

SUBMITTER: Pettersen VK 

PROVIDER: S-EPMC8862486 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature