Transcriptomic comparison of ovarian granulosa cells between adult sheep and prepubertal lambs.

ABSTRACT:

Background

The oocyte development ability of prepubertal animals is significantly lower than that of adult animals. Granulosa cells (GCs) have an important function on regulation of follicular and oocyte development. Therefore, analysis of GC characteristics can be used to explore the developmental mechanism of follicles and oocytes.

Results

In order to understand the possible reasons for the differences in follicle and oocyte development between lambs and adult sheep, we utilized high-throughput sequencing technique to analyze the transcriptome of GCs from follicle-stimulating hormone (FSH) superstimulated adult ewes and prepubertal lambs. Adult ewes were treated with FSH for 3 days (group A) and lambs were FSH-treated for 2 days (group B) or 3 days (group C). Transcriptome

SUBMITTER: Tian H 

PROVIDER: S-EPMC8862527 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature