Gene manipulation in the Mucorales fungus Rhizopus oryzae using TALENs with exonuclease overexpression.

ABSTRACT: The Mucorales fungal genus Rhizopus is used for the industrial production of organic acids, enzymes and fermented foods. The metabolic engineering efficiency of Rhizopus could be improved using gene manipulation; however, exogenous DNA rarely integrates into the host genome. Consequently, a genetic tool for Mucorales fungi needs to be developed. Recently, programmable nucleases that generate DNA double-strand breaks (DSBs) at specific genomic loci have been used for genome editing in various organisms. In this study, we examined gene disruption in Rhizopus oryzae using transcription activator-like effector nucleases (TALENs), with and without exonuclease overexpression. TALENs with an overexpressing exonuclease induced DSBs, followed by target site deletions. Although DSBs are repaired mai

SUBMITTER: Tsuboi Y 

PROVIDER: S-EPMC8863565 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature