Mechanoporation enables rapid and efficient radiolabeling of stem cells for PET imaging.

ABSTRACT: Regenerative medicine uses the patient own stem cells to regenerate damaged tissues. Molecular imaging techniques are commonly used to image the transplanted cells, either right after surgery or at a later time. However, few techniques are fast or straightforward enough to label cells intraoperatively. Adipose tissue-derived stem cells (ADSCs) were harvested from knee joints of minipigs. The cells were labeled with PET contrast agent by flowing mechanoporation using a microfluidic device. While flowing through a series of microchannels, cells are compressed repeatedly by micro-ridges, which open transient pores in their membranes and induce convective transport, intended to facilitate the transport of ⁶⁸Ga-labeled and lipid-coated mesoporous nanoparticles (MSNs) into the cells.

SUBMITTER: Jung KO 

PROVIDER: S-EPMC8863797 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature