High-throughput identification and quantification of single bacterial cells in the microbiota.

ABSTRACT: The bacterial microbiota works as a community that consists of many individual organisms, i.e., cells. To fully understand the function of bacterial microbiota, individual cells must be identified; however, it is difficult with current techniques. Here, we develop a method, Barcoding Bacteria for Identification and Quantification (BarBIQ), which classifies single bacterial cells into taxa-named herein cell-based operational taxonomy units (cOTUs)-based on cellularly barcoded 16S rRNA sequences with single-base accuracy, and quantifies the cell number for each cOTU in the microbiota in a high-throughput manner. We apply BarBIQ to murine cecal microbiotas and quantify in total 3.4 × 10⁵ bacterial cells containing 810 cOTUs. Interestingly, we find location-dependent global differen

SUBMITTER: Jin J 

PROVIDER: S-EPMC8863893 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature