BugSplit enables genome-resolved metagenomics through highly accurate taxonomic binning of metagenomic assemblies.

ABSTRACT: A large gap remains between sequencing a microbial community and characterizing all of the organisms inside of it. Here we develop a novel method to taxonomically bin metagenomic assemblies through alignment of contigs against a reference database. We show that this workflow, BugSplit, bins metagenome-assembled contigs to species with a 33% absolute improvement in F1-score when compared to alternative tools. We perform nanopore mNGS on patients with COVID-19, and using a reference database predating COVID-19, demonstrate that BugSplit's taxonomic binning enables sensitive and specific detection of a novel coronavirus not possible with other approaches. When applied to nanopore mNGS data from cases of Klebsiella pneumoniae and Neisseria gonorrhoeae infection, BugSplit's taxonomic binning ac

SUBMITTER: Chandrakumar I 

PROVIDER: S-EPMC8864044 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature