Determining Sex-Based Differences in Inflammatory Response in an Experimental Traumatic Brain Injury Model.

ABSTRACT:

Introduction

Traumatic brain injury is a leading cause of injury-related death and morbidity. Multiple clinical and pre-clinical studies have reported various results regarding sex-based differences in TBI. Our accepted rodent model of traumatic brain injury was used to identify sex-based differences in the pathological features of TBI.

Methods

Male and female Sprague-Dawley rats were subjected to either controlled-cortical impact (CCI) or sham injury; brain tissue was harvested at different time intervals depending on the specific study. Blood-brain barrier (BBB) analysis was performed using infrared imaging to measure fluorescence dye extravasation. Microglia and splenocytes were characterized with traditional flow cytometry; microglia markers such as CD45, P2Y12, CD32, an

SUBMITTER: Scott MC 

PROVIDER: S-EPMC8864286 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature