Reactions of Arsenoplatin-1 with Protein Targets: A Combined Experimental and Theoretical Study.

ABSTRACT: Arsenoplatin-1 (AP-1) is a dual-action anticancer metallodrug with a promising pharmacological profile that features the simultaneous presence of a cisplatin-like center and an arsenite center. We investigated its interactions with proteins through a joint experimental and theoretical approach. The reactivity of AP-1 with a variety of proteins, including carbonic anhydrase (CA), superoxide dismutase (SOD), myoglobin (Mb), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and human serum albumin (HSA), was analyzed by means of electrospray ionization mass spectrometry (ESI MS) measurements. In accordance with previous observations, ESI MS experiments revealed that the obtained metallodrug-protein adducts originated from the binding of the [(AP-1)-Cl]⁺ fragment to accessible prote

SUBMITTER: Tolbatov I 

PROVIDER: S-EPMC8864615 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature