A complementary chemical probe approach towards customized studies of G-quadruplex DNA structures in live cells.

ABSTRACT: G-quadruplex (G4) DNA structures are implicated in central biological processes and are considered promising therapeutic targets because of their links to human diseases such as cancer. However, functional details of how, when, and why G4 DNA structures form in vivo are largely missing leaving a knowledge gap that requires tailored chemical biology studies in relevant live-cell model systems. Towards this end, we developed a synthetic platform to generate complementary chemical probes centered around one of the most effective and selective G4 stabilizing compounds, Phen-DC3. We used a structure-based design and substantial synthetic devlopments to equip Phen-DC3 with an amine in a position that does not interfere with G4 interactions. We next used this reactive handle to conjugate a

SUBMITTER: Prasad B 

PROVIDER: S-EPMC8864707 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature