In vivo tissue-specific chromatin profiling in Drosophila melanogaster using GFP-tagged nuclei.

ABSTRACT: The chromatin landscape defines cellular identity in multicellular organisms with unique patterns of DNA accessibility and histone marks decorating the genome of each cell type. Thus, profiling the chromatin state of different cell types in an intact organism under disease or physiological conditions can provide insight into how chromatin regulates cell homeostasis in vivo. To overcome the many challenges associated with characterizing chromatin state in specific cell types, we developed an improved approach to isolate Drosophila melanogaster nuclei tagged with a GFPKASH protein. The perinuclear space-localized KASH domain anchors GFP to the outer nuclear membrane, and expression of UAS-GFPKASH can be controlled by tissue-specific Gal4 drivers. Using this protocol, we profiled chromatin ac

SUBMITTER: Jauregui-Lozano J 

PROVIDER: S-EPMC8864739 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature