Mycosis fungoides-derived exosomes promote cell motility and are enriched with microRNA-155 and microRNA-1246, and their plasma-cell-free expression may serve as a potential biomarker for disease burden.

ABSTRACT:

Background

Literature regarding exosomes as mediators in intercellular communication to promote progression in mycosis fungoides (MF) is lacking.

Objectives

To characterize MF-derived exosomes and their involvement in the disease.

Methods

Exosomes were isolated by ultracentrifugation from cutaneous T-cell lymphoma (CTCL) cell lines, and from plasma of patients with MF and controls (healthy individuals). Exosomes were confirmed by electron microscopy, NanoSight and CD81 staining. Cell-line exosomes were profiled for microRNA array. Exosomal microRNA (exomiRNA) expression and uptake, and plasma-cell-free microRNA (cfmiRNA) were analysed by reverse-transcriptase quantitative polymerase chain reaction. Exosome uptake was monitored by fluorescent labelling and CD81 immunos

SUBMITTER: Moyal L 

PROVIDER: S-EPMC8864770 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature