Attenuates of NAD⁺ impair BMSC osteogenesis and fracture repair through OXPHOS.

ABSTRACT:

Background

Controlling the adipo-osteogenic lineage commitment of bone marrow mesenchymal stem cell (BMSC) in favor of osteogenesis is considered a promising approach for bone regeneration and repair. Accumulating evidence indicates that oxidative phosphorylation (OXPHOS) is involved in regulating cell fate decisions. As an essential cofactor for OXPHOS, nicotinamide adenine dinucleotide (NAD) has been shown to correlate with the differentiation of stem cells. However, whether NAD manipulates BMSC lineage commitment through OXPHOS remains elusive. Therefore, it is critical to investigate the potential role of NAD on energy metabolism in mediating BMSC lineage commitment.

Methods

In this study, the mitochondrial respiration and intracellular NAD⁺ level were firstly

SUBMITTER: Li B 

PROVIDER: S-EPMC8864833 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature