Timepoint Selection Strategy for In Vivo Proteome Dynamics from Heavy Water Metabolic Labeling and LC-MS.

ABSTRACT: Protein homeostasis, proteostasis, is essential for healthy cell functioning and is dysregulated in many diseases. Metabolic labeling with heavy water followed by liquid chromatography coupled online to mass spectrometry (LC-MS) is a powerful high-throughput technique to study proteome dynamics in vivo. Longer labeling duration and dense timepoint sampling (TPS) of tissues provide accurate proteome dynamics estimations. However, the experiments are expensive, and they require animal housing and care, as well as labeling with stable isotopes. Often, the animals are sacrificed at selected timepoints to collect tissues. Therefore, it is necessary to optimize TPS for a given number of sampling points and labeling duration and target a specific tissue of study. Currently, such techniques are mi

SUBMITTER: Sadygov VR 

PROVIDER: S-EPMC8864836 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature