SEAseq: a portable and cloud-based chromatin occupancy analysis suite.

ABSTRACT:

Background

Genome-wide protein-DNA binding is popularly assessed using specific antibody pulldown in Chromatin Immunoprecipitation Sequencing (ChIP-Seq) or Cleavage Under Targets and Release Using Nuclease (CUT&RUN) sequencing experiments. These technologies generate high-throughput sequencing data that necessitate the use of multiple sophisticated, computationally intensive genomic tools to make discoveries, but these genomic tools often have a high barrier to use because of computational resource constraints.

Results

We present a comprehensive, infrastructure-independent, computational pipeline called SEAseq, which leverages field-standard, open-source tools for processing and analyzing ChIP-Seq/CUT&RUN data. SEAseq performs extensive analyses from the raw output of the ex

SUBMITTER: Adetunji MO 

PROVIDER: S-EPMC8864840 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature