METTL3/IGF2BP3 axis inhibits tumor immune surveillance by upregulating N⁶-methyladenosine modification of PD-L1 mRNA in breast cancer.

ABSTRACT:

Background

Continual expression of PD-L1 in tumor cells is critical for tumor immune escape and host T cell exhaustion, however, knowledge on its clinical benefits through inhibition is limited in breast cancer. N⁶-methyladenosine (m⁶A) plays a crucial role in multiple biological activities. Our study aimed to investigate the regulatory role of the m⁶A modification in PD-L1 expression and immune surveillance in breast cancer.

Methods

MeRIP-seq and epitranscriptomic microarray identified that PD-L1 is the downstream target of METTL3. MeRIP-qPCR, absolute quantification of m⁶A modification assay, and RIP-qPCR were used to examine the molecular mechanism underlying METTL3/m⁶A/IGF2BP3 signaling axis in PD-L1 expression. B-ND

SUBMITTER: Wan W 

PROVIDER: S-EPMC8864846 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature