Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate.

ABSTRACT:

Background

Butyl acetate is a versatile compound that is widely used in the chemical and food industry. The conventional butyl acetate synthesis via Fischer esterification of butanol and acetic acid using catalytic strong acids under high temperature is not environmentally benign. Alternative lipase-catalyzed ester formation requires a significant amount of organic solvent which also presents another environmental challenge. Therefore, a microbial cell factory capable of producing butyl acetate through fermentation of renewable resources would provide a greener approach to butyl acetate production.

Result

Here, we developed a metabolically engineered strain of Escherichia coli that efficiently converts glucose to butyl acetate. A modified Clostridium CoA-dependent butanol pr

SUBMITTER: Ku JT 

PROVIDER: S-EPMC8864926 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature