A Single-Organelle Optical Omics Platform for Cell Science and Biomarker Discovery.

ABSTRACT: Research in fundamental cell biology and pathology could be revolutionized by developing the capacity for quantitative molecular analysis of subcellular structures. To that end, we introduce the Ramanomics platform, based on confocal Raman microspectrometry coupled to a biomolecular component analysis algorithm, which together enable us to molecularly profile single organelles in a live-cell environment. This emerging omics approach categorizes the entire molecular makeup of a sample into about a dozen of general classes and subclasses of biomolecules and quantifies their amounts in submicrometer volumes. A major contribution of our study is an attempt to bridge Raman spectrometry with big-data analysis in order to identify complex patterns of biomolecules in a single cellular organelle an

SUBMITTER: Pliss A 

PROVIDER: S-EPMC8865453 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature