Slow nucleosome dynamics set the transcriptional speed limit and induce RNA polymerase II traffic jams and bursts.

ABSTRACT: Nucleosomes are recognized as key regulators of transcription. However, the relationship between slow nucleosome unwrapping dynamics and bulk transcriptional properties has not been thoroughly explored. Here, an agent-based model that we call the dynamic defect Totally Asymmetric Simple Exclusion Process (ddTASEP) was constructed to investigate the effects of nucleosome-induced pausing on transcriptional dynamics. Pausing due to slow nucleosome dynamics induced RNAPII convoy formation, which would cooperatively prevent nucleosome rebinding leading to bursts of transcription. The mean first passage time (MFPT) and the variance of first passage time (VFPT) were analytically expressed in terms of the nucleosome rate constants, allowing for the direct quantification of the effects of nucleosom

SUBMITTER: Mines RC 

PROVIDER: S-EPMC8865691 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature