Protocol for chronic hepatitis B virus infection mouse model development by patient-derived orthotopic xenografts.

ABSTRACT:

Background

According to the World Health Organization, more than 250 million people worldwide are chronically infected with the hepatitis B virus, and almost 800.000 patients die annually of mediated liver disorders. Therefore, adequate biological test systems are needed that could fully simulate the course of chronic hepatitis B virus infection, including in patients with hepatocellular carcinoma.

Methods

In this study, we will assess the effectiveness of existing protocols for isolation and cultivation of primary cells derived from patients with hepatocellular carcinoma in terms of the yield of viable cells and their ability to replicate the hepatitis B virus using isolation and cultivation methods for adhesive primary cells, flow cytometry and quantitative polymerase chai

SUBMITTER: Nagornykh AM 

PROVIDER: S-EPMC8865695 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature