Mg²⁺-dependent conformational equilibria in CorA and an integrated view on transport regulation.

ABSTRACT: The CorA family of proteins regulates the homeostasis of divalent metal ions in many bacteria, archaea, and eukaryotic mitochondria, making it an important target in the investigation of the mechanisms of transport and its functional regulation. Although numerous structures of open and closed channels are now available for the CorA family, the mechanism of the transport regulation remains elusive. Here, we investigated the conformational distribution and associated dynamic behaviour of the pentameric Mg²⁺ channel CorA at room temperature using small-angle neutron scattering (SANS) in combination with molecular dynamics (MD) simulations and solid-state nuclear magnetic resonance spectroscopy (NMR). We find that neither the Mg²⁺-bound closed structure nor the Mg^2+<

SUBMITTER: Johansen NT 

PROVIDER: S-EPMC8865849 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature