PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells.

ABSTRACT: Prime editing is a recently developed CRISPR/Cas9 based gene engineering tool that allows the introduction of short insertions, deletions, and substitutions into the genome. However, the efficiency of prime editing, which typically achieves editing rates of around 10%-30%, has not matched its versatility. Here, we introduce the prime editor activity reporter (PEAR), a sensitive fluorescent tool for identifying single cells with prime editing activity. PEAR has no background fluorescence and specifically indicates prime editing events. Its design provides apparently unlimited flexibility for sequence variation along the entire length of the spacer sequence, making it uniquely suited for systematic investigation of sequence features that influence prime editing activity. The use of PEAR as a

SUBMITTER: Simon DA 

PROVIDER: S-EPMC8865850 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature