Dataset Information

CircRNA expression in chicken granulosa cells illuminated with red light.

ABSTRACT: Red light (RL) can improve egg production in Jinghai Yellow hens. Circular RNAs (circRNAs) are novel, non-coding RNAs, but the molecular mechanism underlying circRNA function during follicular development in hens under monochromatic light has not been established. Herein, we compared expression profiles of granulosa cells (GCs) from small yellow follicles (SYFs) from hens under RL and white light (WL). A total of 2,468 circRNAs were identified, of which 22 were differentially expressed (DE) in the RL and WL groups. DE circRNA host genes were enriched in ovarian steroidogenesis, and MAPK and PI3K-Akt signaling pathways. Furthermore, DE circRNA_0320 and circRNA_0185 interacted with miR-143-3p, which targets the follicle-stimulating hormone receptor and is essential for GC differentiation and follicle development. These findings will facilitate further analysis of the molecular mechanism leading to GC development in hens raised under monochromatic light, which could lead to increased egg production.

SUBMITTER: Wang Y

PROVIDER: S-EPMC8866055 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

CircRNA expression in chicken granulosa cells illuminated with red light.

Wang Ying Y Guo Zhenyu Z Zi Chen C Wu Pengfei P Lv Xiaoyang X Chen Lan L Chen Fuxiang F Zhang Genxi G Wang Jinyu J

Poultry science 20220117 4

Red light (RL) can improve egg production in Jinghai Yellow hens. Circular RNAs (circRNAs) are novel, non-coding RNAs, but the molecular mechanism underlying circRNA function during follicular development in hens under monochromatic light has not been established. Herein, we compared expression profiles of granulosa cells (GCs) from small yellow follicles (SYFs) from hens under RL and white light (WL). A total of 2,468 circRNAs were identified, of which 22 were differentially expressed (DE) in t ...[more]

PMID: 35202895

Similar Datasets

Project description:In this study, we aimed to better understand the difference between the functions of the two types of granulosa cells and sought to discover more key genes involved in follicle development and follicle selection. Herein, we separately collected pre-hierarchical follicle granulosa cells (PHGCs) and preovulatory follicle granulosa cells (POGCs) for RNA extraction; the transcriptomes of the two groups were compared via RNA-seq. A total of 5273 differentially expressed genes (DEGs) were identified between the PHGCs and POGCs; 2797 genes were up-regulated and 2476 were down-regulated in the PHGCs compared with the POGCs. A qPCR analysis confirmed that the expression patterns of 16 randomly selected DEGs were highly consistent with the RNA-seq results. In the POGCs, many of the genes with the most significant increase in expression were related to steroid hormone synthesis. In addition, the genes with the most significant decline in expression, including AMH and WT1, were related to the inhibition of steroid hormone synthesis. These results suggest that steroid hormones play a key role in follicle development. Furthermore, a Gene Ontology (GO) analysis revealed that these DEGs were mainly involved in the primary metabolic process, the carbohydrate metabolic process, the cellular process, ribosomes, the cytoplasm, and intracellular processes. A Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the DEGs were mainly enriched in steroid biosynthesis, the cell cycle, ribosomes, the TGF-beta signaling pathway, focal adhesion, and so on. We also observed the morphology of the follicles at different developmental stages, and the results showed that the thickness of the granular layer of the small yellow follicles (SYFs) decreased significantly with further development. In addition, we also found that the thickness of the granulosa layer of hens over 300 days old was significantly lower than that of 200-day-old hens. In short, these data indicate that the tissue morphology and function of granulosa cells change throughout follicle development.

Dataset Information

CircRNA expression in chicken granulosa cells illuminated with red light.

Publications

CircRNA expression in chicken granulosa cells illuminated with red light.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets