Paracrine Wnt signaling is necessary for prostate epithelial proliferation.

ABSTRACT:

Introduction

The Wnt proteins play key roles in the development, homeostasis, and disease progression of many organs including the prostate. However, the spatiotemporal expression patterns of Wnt proteins in prostate cell lineages at different developmental stages and in prostate cancer remain inadequately characterized.

Methods

We isolated the epithelial and stromal cells in the developing and mature mouse prostate by flow cytometry and determined the expression levels of Wnt ligands. We used Visium spatial gene expression analysis to determine the spatial distribution of Wnt ligands in the mouse prostatic glands. Using laser-capture microscopy in combination with gene expression analysis, we also determined the expression patterns of Wnt signaling components in stromal and

SUBMITTER: Wei X 

PROVIDER: S-EPMC8866211 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature