An ex vivo organ culture screening model revealed that low temperature conditions prevent side effects of anticancer drugs.

ABSTRACT: Development of chemotherapy has led to a high survival rate of cancer patients; however, the severe side effects of anticancer drugs, including organ hypoplasia, persist. To assume the side effect of anticancer drugs, we established a new ex vivo screening model and described a method for suppressing side effects. Cyclophosphamide (CPA) is a commonly used anticancer drug and causes severe side effects in developing organs with intensive proliferation, including the teeth and hair. Using the organ culture model, we found that treatment with CPA disturbed the growth of tooth germs by inducing DNA damage, apoptosis and suppressing cellular proliferation and differentiation. Furthermore, low temperature suppressed CPA-mediated inhibition of organ development. Our ex vivo and in vitro analysis

SUBMITTER: Tian T 

PROVIDER: S-EPMC8866511 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature