Electric-field induced modulation of amorphous protein aggregates: polarization, deformation, and reorientation.

ABSTRACT: Proteins in their native state are only marginally stable and tend to aggregate. However, protein misfolding and condensation are often associated with undesired processes, such as pathogenesis, or unwanted properties, such as reduced biological activity, immunogenicity, or uncontrolled materials properties. Therefore, controlling protein aggregation is very important, but still a major challenge in various fields, including medicine, pharmacology, food processing, and materials science. Here, flexible, amorphous, micron-sized protein aggregates composed of lysozyme molecules reduced by dithiothreitol are used as a model system. The preformed amorphous protein aggregates are exposed to a weak alternating current electric field. Their field response is followed in situ by time-resolved pola

SUBMITTER: Kang K 

PROVIDER: S-EPMC8866516 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature