Altered circadian clock gene expression in the sperm of infertile men with asthenozoospermia.

ABSTRACT:

Purpose

Male infertility is a complex multifactorial pathological condition, and asthenozoospermia (AZS) is one of the most common causes. Current evidence suggests the underlying role of the circadian clock on male fertility. This study aims to evaluate the expression levels of five principal clock genes in the sperm and their correlations with the sperm parameters in male infertility.

Methods

We determined the expression profiles of BMAL1, CLOCK, CRY1, PER1, and PER2 in the sperm of infertile men with AZS (n=38) and healthy fertile men (n=40) using quantitative real-time PCR. Then we performed comprehensive association analyses on the clock gene levels and the sperm parameters, including progressive and total motility, concentration, and normal morphology of the sperm.

SUBMITTER: Zhang P 

PROVIDER: S-EPMC8866580 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature