Desiccated cat spermatozoa retain DNA integrity and developmental potential after prolonged storage and shipping at non-cryogenic temperatures.

ABSTRACT:

Purpose

To evaluate the DNA integrity and developmental potential of microwave-dehydrated cat spermatozoa after storage at - 20 °C for different time periods and/or overnight shipping on dry ice.

Methods

Epididymal spermatozoa from domestic cats were microwave-dehydrated on coverslips after trehalose exposure. Dried samples were either assessed immediately, stored for various duration at - 20 °C, or shipped internationally on dry ice before continued storage. Dry-stored spermatozoa were rehydrated before assessing DNA integrity (TUNEL assays) or developmental potential (injection into in vitro matured oocytes followed by in vitro embryo culture for up to 7 days).

Results

Percentages of dried-rehydrated spermatozoa with intact DNA was not significantly affected (P > 0.

SUBMITTER: Lee PC 

PROVIDER: S-EPMC8866589 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature