Morphometric and morphokinetic differences in the sperm- and oocyte-originated pronuclei of male and female human zygotes: a time-lapse study.

ABSTRACT:

Purpose

To study the morphometric and morphokinetic profiles of pronuclei (PN) between male and female human zygotes.

Method(s)

This retrospective cohort study included 94 consecutive autologous single day 5 transfer cycles leading to a singleton live birth. All oocytes were placed in the EmbryoScope + incubator post-sperm injection with all annotations performed retrospectively by one embryologist (L-SO). Timing parameters included 2nd polar body extrusion (tPB2), sperm-originated PN (tSPNa) or oocyte-originated PN (tOPNa) appearance, and PN fading (tPNF). Morphometrics were evaluated at 8 (stage 1), 4 (stage 2), and 0 h before PNF (stage 3), measuring PN area (um²), PN juxtaposition, and nucleolar precursor bodies (NPB) arrangement.

Results

Male zygotes h

SUBMITTER: Orevich LS 

PROVIDER: S-EPMC8866592 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature