GDF9 concentration in embryo culture medium is linked to human embryo quality and viability.

ABSTRACT:

Purpose

We aimed to evaluate the link between the GDF9 concentration in day 3 human embryo culture medium and embryo quality and viability.

Methods

Two independent, prospective, observational studies were conducted. In study 1, a total of 280 embryos from 70 patients who obtained at least 4 embryos with 6-10 blastomeres (2 transferable and 2 non-transferable embryos) at day 3 were enrolled. In study 2, a total of 119 embryos from 61 patients (29 fully implanted and 32 non-implanted patients) were enrolled. The corresponding GDF9 concentrations in spent culture medium of embryos were quantified by ELISA assay. The expression pattern of GDF9 in human embryos was investigated using Q-PCR and immunofluorescence.

Results

GDF9 mRNA and protein were detected from human oocyt

SUBMITTER: Li J 

PROVIDER: S-EPMC8866627 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature