Effect of cumulin and super-GDF9 in standard and biphasic mouse IVM.

ABSTRACT:

Purpose

In vitro maturation (IVM) is a technology that generates mature oocytes following culture of immature cumulus-oocyte complexes (COC) in vitro. IVM is characterized by minimal patient stimulation, making it attractive for certain patient groups. Recently, a biphasic IVM system, capacitation (CAPA)-IVM, has shown improved clinical outcomes relative to standard IVM; however, it remains less efficient than IVF. This study assessed whether supplementation of CAPA-IVM culture media with the novel TGFβ superfamily proteins cumulin and super-GDF9 improves subsequent mouse embryo development.

Methods

Immature mouse COCs were cultured by standard IVM or biphasic IVM ± cumulin or super-GDF9.

Results

Both cumulin and super-GDF9 in standard IVM significantly improved day-6

SUBMITTER: Akin N 

PROVIDER: S-EPMC8866628 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature