The function of Nucleoporin 37 on mouse oocyte maturation and preimplantation embryo development.

ABSTRACT:

Purpose

Nucleoporin 37 (NUP37) has been reported to activate the YAP-TEAD signaling, which is crucial for early embryo development. However, whether NUP37 is involved in oocyte meiosis and embryo development remains largely unknown. The study aimed to clarify the function of Nup37 in oocyte maturation and early embryo development, and to explore the mechanism.

Methods

The expression level and subcellular localization of NUP37 were explored. After knocking down of Nup37 by microinjecting interfering RNA (siRNA), the oocyte maturation rate, aberrant PB1 extrusion rate, and blastocyst formation rate were evaluated. In addition, the effect of the downregulation of Nup37 on YAP-TEAD signaling was confirmed by immunofluorescence staining and real-time quantitative PCR.

Results<

SUBMITTER: Guo Q 

PROVIDER: S-EPMC8866631 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature