Crosstalk between TM4SF5 and GLUT8 regulates fructose metabolism in hepatic steatosis.

ABSTRACT:

Objective

Transmembrane 4 L six family member 5 (TM4SF5) is likely involved in non-alcoholic steatohepatitis, although its roles and cross-talks with glucose/fructose transporters in phenotypes derived from high-carbohydrate diets remain unexplored. Here, we investigated the modulation of hepatic fructose metabolism by TM4SF5.

Methods

Wild-type or Tm4sf5^-/- knockout mice were evaluated via different diets, including normal chow, high-sucrose diet, or high-fat diet without or with fructose in drinking water (30% w/v). Using liver tissues and blood samples from the mice or hepatocytes, the roles of TM4SF5 in fructose-mediated de novo lipogenesis (DNL) and steatosis via a crosstalk with glucose transporter 8 (GLUT8) were assessed.

Results

Tm4sf5 suppression or

SUBMITTER: Lee H 

PROVIDER: S-EPMC8866669 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature