The data of an all-solid-state acupuncture needle based potentiometric microelectrode for in vivo monitoring of calcium ions in rat cerebrospinal fluid.

ABSTRACT: These data contain the details of the fabrication of the calcium ion-selective microelectrode (Ca²⁺-ISμEs) modified with poly(3,4-ethylenedioxythiophene)-poly(sodium 4-styrenesulfonate) (PEDOT(PSS)) as solid contact. The electrochemical impedance spectroscopy was carried out for the investigation of the resistance of the Ca²⁺-ISμEs. The thickness of the solid contact and the calcium ion-selective membrane was investigated by SEM. Potential-time curve of the electrodeposition of the PEDOT/PSS film onto the surface of the microelectrodes under the applied current of 0.5 μA for 200 s was recorded. The proposed Ca²⁺-ISμE was optimized through conditioning in different CaCl₂ solutions ranged from 1.0 × 10^-6 to 3.1 × 10^-3 M for different time before use. The anti-fouling property of the Ca²⁺-ISμEs against proteins was investigated through taking BSA as the model protein. The developed Ca²⁺-ISμEs were used for the in vivo monitoring of the calcium ions in rat cerebrospinal fluid under the stimuli of the spinal cord transection in a living animal.

SUBMITTER: Zhai J 

PROVIDER: S-EPMC8866674 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature